Journal: Frontiers in Nutrition

Article Title: Fructose Induces Visceral Adipose Tissue Inflammation and Insulin Resistance Even Without Development of Obesity in Adult Female but Not in Male Rats

doi: 10.3389/fnut.2021.749328

Figure Lengend Snippet: Inflammation in the VAT of female rats fed with 10% fructose diet for 9 weeks. Protein level of NFκB in (A) cytosols and (B) nucleosols, and protein level of (C) F4/80 in whole cell extracts were measured by Western blot in the VAT from control (C) and fructose-fed (F) rats, normalized to β actin and expressed in AUs. The values represent the means ± SEM. Statistical significance of the difference between experimental groups (Student's t -test): ** P < 0.01 and *** P < 0.001, F vs. C. The level of TNFα, IL-1β, and IL-6 mRNAs relative to HPRT mRNA were determined by TaqMan real-time PCR in the VAT (D) . The values represent the mean ± SEM ( n = 8 animals per group). All measurements were done in triplicate. Statistical significance of the difference between experimental groups (Student's t -test): ** P < 0.01 and *** P < 0.001, F vs. C.

Article Snippet: Anti-SOD1 (ab13498), anti-SOD2 (ab13533), anti-GRed (ab16801), anti-catalase (ab16731) and anti-GPx (ab22604) primary antibodies, and secondary anti-mouse (ab97046), anti-rabbit (ab6721) and anti-goat (ab6741) IgG H and L horseradish peroxidase (HRP)-linked antibody were obtained from Abcam (Cambridge, United Kingdom); anti-NFκB/p65 (sc-372), anti-phospho-Akt 1/2/3 (Ser473, sc-7985-R), anti-Akt 1/2/3 (sc-8312), anti-phospho-IRS-1 (Ser307, sc-33956), anti-IRS1 (E-12; sc-8038), anti-PTP1B (N-19, sc-1718-R), anti-lamin B (M-20; sc-6217), anti-F4/80 (sc-26643-R), anti-PPARγ antibody (sc-7273) were purchased from Santa Cruz Biotechnology (Dallas, TX, United States), and anti-β-actin antibody (AC 15) was purchased from Sigma Chemicals (St. Louis, MO, United States).

Techniques: Western Blot, Real-time Polymerase Chain Reaction

Journal: Cell chemical biology

Article Title: A Strategic Approach for Fluorescence Imaging of Membrane Proteins in a Native-Like Environment

doi: 10.1016/j.chembiol.2019.11.008

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: The pZE21-GFPaav vector was a gift from Prof. Michael Elowitz (Addgene plasmid # 26643; http://n2t.net/addgene:26643 ; RRID: Addgene_26643).

Techniques: Recombinant, Plasmid Preparation, Mutagenesis, Sequencing, Expressing, Software